Coding
Part:BBa_K1486000:Design
Designed by: iGem EPFL 2014 Group: iGEM14_EPF_Lausanne (2014-08-12)
CpxR coding region
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 61
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 472
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
When making composite parts with CpxR that are supposed to dimerize, we had to take care about the positioning of the other elements regarding the parallel dimerization of CpxR. Its DNA binding domain was also taken into account for the design, as it shouldn't be blocked by the attached parts. Also, stop and start codons have been added or removed to meet the needs of our composite parts.
Source
The part has been extracted from the E coli genome.