Coding

Part:BBa_K1486000:Design

Designed by: iGem EPFL 2014   Group: iGEM14_EPF_Lausanne   (2014-08-12)

CpxR coding region


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 61
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 472
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

When making composite parts with CpxR that are supposed to dimerize, we had to take care about the positioning of the other elements regarding the parallel dimerization of CpxR. Its DNA binding domain was also taken into account for the design, as it shouldn't be blocked by the attached parts. Also, stop and start codons have been added or removed to meet the needs of our composite parts.

Source

The part has been extracted from the E coli genome.

References